Martinez SR, Ravetti S, Aciar RMA, Becerra MC.. Antimicrobial Activity of S-(-)-Perillyl Aldehyde in Staphylococcus aureus and Escherichia coli.

MARTINEZ SR; RAVETTI S; ACIAR RMA; BECERRA MC

Rosario

Congreso; RICiFa 2° Reunión Internacional de Ciencias Farmacéuticas; 2012

Fac. Ciencias Químicas-UNC y la Univ. Nac. de Rosario

Antimicrobial Activity of S-(-)-Perillyl Aldehyde in Staphylococcus aureus and Escherichia coli Martinez SR1, Ravetti S1, Aciar RMA2, Becerra MC1 1- Depto. de Farmacia. IMBIV-CONICET. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. Córdoba, Argentina. CP. 5000. E-mail: becerra@fcq.unc.edu.ar 2- Facultad de Ciencias de la Alimentación, Bioquímicas y Farmacéuticas. Universidad Católica de Cuyo. San Juan, Argentina. CP. 5400.   Introducción S-(-)-Perillaldehyde (4-isopropenylcyclohex-1-ene-1-carbaldehyde, PALD), is a monoterpene, a secondary metabolite and an atmospheric pollutant obtained from the oxidation of other terpenes, as limonene and α-pinene, among others. PALD is a relatively non-toxic agent that has been used in pre-clinical testing because of their therapeutic properties and chemopreventive activity against a wide variety of cancers.1 Objectives In this study we examined the antimicrobial activities of PALD in bacterial species, including clinical and reference strains of Escherichia coli and Staphylococcus aureus. Materials and Methods Antimicrobial activity of Perillyl aldehyde was determined in Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, extended beta-lactamase producing E.coli (BLEE) and S.aureus methicillin resistant by microdilution method. 2 Suspensions of each strain were incubated in Mueller Hinton broth (MHB) at 37 °C during 24 h. The final concentration was adjusted to 5x105 CFU/mL (colonies forming unities per mL). A stock solution of PALD was solubilized in polyethyleneglycol (PEG) 400 and ethyl alcohol at a 7:3 ratio. The concentration of the stock solution of PALD was 64.2 mM and the final concentration of the solvent was lower than 10% for all the experiments. Serial dilutions of the essential oil over a range of 0.12 to 2.40 µg/mL were prepared. Minimum inhibitory concentration (MIC) was defined as the lowest concentration of an essential oil at which the microorganism does not exhibit visible growth, as indicated by the turbidity of the medium.  The drug-free control medium contained similar concentrations of PEG and ethyl alcohol (bacterial cultures treated with solvent alone). Results Gram-negative bacteria (E. coli) and Gram-positive bacteria (S. aureus) were employed as screening microorganisms. For E. coli strains, the essential oil exhibited inhibitory activity against at 0.96 mg/mL and the inhibitory activity against the reference and the clinical strains of S. aureus was obtained at 1.44 mg/mL. Gram-positive organisms are slightly less susceptible than Gram-negative bacteria. Conclusion Extracts of fruits of Kefe cumin (Laser trilobum L.) obtained from different regions (Germany, India, and Turkey), contained perillaldehyde (4.23–32.75%) had demonstrated a significant antimicrobial effect on different pathogen bacteria.3 This investigation corroborates the monoterpene PALD as an effective antimicrobial agent. References 1- Elegbede JA, Flores R, Wang RC. Perillyl alcohol and perillaldehyde induced cell cycle arrest and cell death in BroTo and A549 cells cultured in vitro. Life Sci. (2003) 73(22), 2831-40. 2- Tsai ML, Lin CC, Lin WC, Yang CH. Antimicrobial, Antioxidant and Anti-Inflammatory Activities of Essentials Oils from Five Selected Herbs. Biosci. Biotechnol. Biochem. (2011) 75(10), 1977-1983. 3- Parlatan A, Sariçoban C, Ozcan MM. Chemical composition and antimicrobial activity of the extracts of Kefe cumin (Laser trilobum L.) fruits from different regions. Int. J. Food Sci. Nutr. (2009) 60(7), 606-17.