Sistema para la cría de larvas de Haematobia irritans (Diptera: Muscidae) a partir 3 de ejemplares salvajes de Argentina

JACQUELIN D; FILIBERTI, ADRIAN; ARGARAÑA, CARLOS, C.A; RABOSSI A

REVISTA DE LA SOCIEDAD ENTOMOLóGICA ARGENTINA

SOCIEDAD ENTOMOLÓGICA ARGENTINA

Lugar: Mendoza; Año: 2016 vol. 75 p. 153 - 159

0373-5680

ABSTRACT. A simple laboratory system to sustain the development of the larva to adult 22 stage of wild-type horn fly, Haematobia irritans (L.) is described. The culture medium is 23 based on a bacterial suspension obtained from bovine fecal feces (olidic diet) and a support material consisting of cotton gauze where the bacterial suspension is deposited. The conditions for optimal bacterial extraction as well as conditions for its preservation are described. The optimal concentration of bacterial suspension that supports the development of a fixed number of larvae was determined. At this concentration, the percentage of larvae alive at 48 h was 86.5% and 68.5% (96 h) of the initial number of Larvae seeded could pupate and initiate metamorphosis. Comparable results were obtained (93 and 75% respectively) using bovine fecal feces as a culture medium. Since the medium consisted of a bacterial suspension, allows an easy visual inspection of thelarvae to study its developmental stages, and it also permits the bioassay of soluble insecticidal compounds. The construction and operating parameters of a breeding chamber, which allows incubation of the culture media at a controlled temperature and humidity, are also described in this work.