PMCA activity and membrane tubulin affect deformability of erythrocytes from normal and hypertensive human subjects.

MONESTEROLO N.E.; NIGRA AD; CAMPETELLI A.N.; SANTANDER V.S.; RIVELLI J.F.; ARCE C.A.; CASALE C.H.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 1848 p. 2813 - 2820

0005-2736

Our previous studies demonstrated formation of a complex between acetylated tubulin and brain plasmamembrane Ca2+-ATPase (PMCA), and the effect of the lipid environment on structure of this complex and onPMCA activity. Deformability of erythrocytes from hypertensive human subjects was reduced by an increase inmembrane tubulin content. In the present study, we examined the regulation of PMCA activity by tubulin innormotensive and hypertensive erythrocytes, and the effect of exogenously added diacylglycerol (DAG) andphosphatidic acid (PA) on erythrocyte deformability. Some of the key findings were that: (i) PMCA was associatedwith tubulin in normotensive and hypertensive erythrocytes, (ii) PMCA enzyme activity was directly correlatedwith erythrocyte deformability, and (iii) when tubulin was present in the erythrocyte membrane, treatmentwith DAG or PA led to increased deformability and associated PMCA activity. Taken together, our findings indicatethat PMCA activity is involved in deformability of both normotensive and hypertensive erythrocytes. This rheologicalproperty of erythrocytes is affected by acetylated tubulin and its lipid environment because both regulatePMCA activity.