CIQUIBIC   05472
CENTRO DE INVESTIGACIONES EN QUIMICA BIOLOGICA DE CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Content of Endoplasmic Reticulum and Golgi Complex Membranes Positively Correlate with the Proliferative Status of brain Cells
Autor/es:
SILVESTRE DC; MACCIONI HJF; CAPUTTO BL
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
Wiley InterScience
Referencias:
Lugar: Hoboken, New Jersey; Año: 2008 vol. 87 p. 857 - 865
ISSN:
0360-4012
Resumen:
Although the molecular and cellular basis of particular
events that lead to the biogenesis of membranes in eukaryotic
cells has been described in detail, understanding
of the intrinsic complexity of the pleiotropic
response by which a cell adjusts the overall activity of
its endomembrane system to accomplish these requirements
is limited. Here we carried out an immunocytochemical
and biochemical examination of the content
and quality of the endoplasmic reticulum (ER) and Golgi
apparatus membranes in two in vivo situations characterized
by a phase of active cell proliferation followed
by a phase of declination in proliferation (rat brain tissue
at early and late developmental stages) or by permanent
active proliferation (gliomas and their most malignant
manifestation, glioblastomas multiforme). It was
found that, in highly proliferative phases of brain development
(early embryo brain cells), the content of ER
and Golgi apparatus membranes, measured as total
lipid phosphorous content, is higher than in adult brain
cells. In addition, the concentration of protein markers
of ER and Golgi is also higher in early embryo brain
cells and in human glioblastoma multiforme cells than
in adult rat brain or in nonpathological human brain
cells. Results suggest that the amount of endomembranes
and the concentration of constituent functional
proteins diminish as cells decline in their proliferative
activity. VVC 2008 Wiley-Liss, Inc.VVC 2008 Wiley-Liss, Inc.