Staining tumor cells with biotinylated ACL-I, a lectin isolated from the marine sponge, Axinella corrugata

DRESCH RR; ZANETTI GD; IRAZOQUI FJ; SENDRA VG; ZLOCOWSKI N; BERNARDI A; ROSA RM; BATTASTINI AM; HENRIQUES AT; VOZÁRI-HAMPE MM

BIOTECHNIC AND HISTOCHEMISTRY

INFORMA HEALTHCARE

Lugar: London; Año: 2013 p. 1 - 9

1052-0295

Axinella corrugata lectin 1 (ACL-1) was purifi ed from aqueous extracts of the marine sponge,Axinella corrugata . ACL-1 strongly agglutinates native rabbit erythrocytes. The hemagglutinationis inhibited by N -acetyl derivatives, particularly N , N ’ , N ” -triacetylchitotriose, N -acetyl-Dglucosamine,N -acetyl-D-mannosamine and N -acetyl-D-galactosamine. We investigated the capacityof biotinylated ACL-1 to stain several transformed cell lines including breast (T-47D, MCF7),colon (HT-29), lung (H460), ovary (OVCAR-3) and bladder (T24). ACL-I may bind to both monosaccharidesand oligosaccharides of tumor cells, N -acetyl-D-galactosamine, and N -acetyl-Dglucosamineglycan types. The lectins are useful, not only as markers and diagnostic parameters,but also for tissue mapping in suspicious neoplasms. In addition, they provide a better understandingof neoplasms at the cytological and molecular levels. Furthermore, the use of potentialmetastatic markers such as lectins is crucial for developing successful tools for therapy againstcancer. We observed that biotinylated ACL-I stains tumor cells and may hold potential as a probefor identifying transformed cells and for studying glycan structures synthesized by such cells.