CONICET | Buscador de Institutos y Recursos Humanos

Ghrelin (Ghr) is a gut / hypothalamus peptide with inhibitory actions on reproductive physiology; however, there are no previous reports of its role on estrous behavior. Under the hypothesis that the increase of plasma Ghr during food restriction (FR) is responsible for receptivity reduction, we intended to evaluate the receptivity percentage of female mice subjected to: exp. 1) acute and chronic FR and Ghr administration (3 nmol / animal / day, s. c.) and exp. 2) the co-administration of a ghrelin antagonist [ant = (D-Lys3)-GHRP-6; 6 nmol / animal / day s. c.]. All females were ovariectomized, primed with steroids, trained, and randomly subjected every week to each one of several protocols, followed by a behavioral test. Experiment 1 (n = 8): basal, no treatment; acute FR (aFR), 24-h fasting; chronic FR (cFR), 50 % FR for 5 days; acute ghrelin (aGhr), Ghr 30 min before test and chronic ghrelin (cGhr), Ghr for 5 days. Except for cGhr, all treatments signifi cantly decreased the percentage of receptivity (mean ± SEM): basal 61.9 ± 6.0, aFR 33.1 ± 8.1, cFR 18.8 ± 7.7, aGhr 45.6 ± 10.6, p < 0.05 vs. basal. In exp. 2 (n = 11), except for cFR + ant (55.0 ± 6.4) the co-administration of the antagonist reversed the deleterious eff ects detected in exp. 1: basal 70.9 ± 5.4; aFR + ant 72.3 ± 7.6; aGhr + ant 73.6 ± 4.7. As expected, the administration of vehicle or antagonist alone did not modify receptivity. Besides, we found a signifi cant correlation between percentage of body weight loss and percentage of receptivity reduction (r = 0.62, p = 0.0004). This is the fi rst study demonstrating that ghrelin is able to inhibit female mice sexual behavior and that is involved, at least in part, in receptivity reduction after food scarcity.