hiPSC- and hNPC-derived Extracellular Vesicles: composition and biological activity

HERRERA LÓPEZ, MALENA; CESCHIN, DANILO; GASTALDI, LAURA; MOYANO, ANA LIS; REMEDI, MÓNICA; CÁCERES, ALFREDO

Carlos Paz

Congreso; XXXIV Annual Meeting of the Argentine Society for Research in Neuroscience; 2019

Sociedad Argentina de Investigación en Neurociencias

Extracellular vesicles (EV) are nanovesicles (50-200 nm) released to the extracellular microenvironment by most cell types. EV regulate biological processes by transferring lipids, proteins and miRNA cargo between cells during physiological and pathological conditions. Given their ability to transfer bioactive components and their biocompatibility, EV are increasingly being explored as potential therapeutic agents. Stem cell-derived EV promote endogenous regenerative mechanisms and functional recovery in animal models of the central nervous system (CNS). Whether EV secreted by human induced pluripotent stem cells (hiPSC) and human neural precursor cells (hNPC) might have similar therapeutic potential in CNS diseases is still unknown. Moreover, the molecular and cellular mechanisms responsible for their potential regenerative and immunomodulatory effects are unclear. Here, we used a combination of in silico systems biology, biochemical and optical approaches to evaluate compositional and morphological differences between EV secreted by hNPC and hiPSC. These results will help us to shed some light on how stem cell-derived EV might exert their regenerative effects and improve their therapeutic potential in CNS disorders.