THE EFFECT OF MONO- OR BIPARENTAL CARE ON ALCOHOL-INDUCED MOTOR STIMULATION AND NEURAL ACTIVATION IN INFANT MICE.

PASQUETTA, LUCILA; RAMIREZ, ABRAHAM; FERREYRA, ELIANA; WILLE-BILLE, ARANZA; MIRANDA-MORALES, ROBERTO SEBASTIÁN

Córdoba

Conferencia; IX INTERNATIONAL MEETING of the Latin American Society for Biomedical Research on Alcoholism (LASBRA); 2019

LASBRA

Social experiences play an important role in offspring development. We have evidenced that monoparental (ie., single mother) rearing condition in C57BL/6 mice induced an anxiety-like behavior and major alcohol consumption in adolescent offspring. Here, we aimed to analyze if mono- or biparental conditions could alter alcohol-induced locomotor stimulation and its effects on neural activation in several brain areas. Infant mice were reared in a monoparental (MP, single mother) or biparental (BP, father and mother) condition. At postnatal days (PD) 16, 17, 18 and 20, infants were administered with a 0.0 or 2.0 g/kg alcohol dose and evaluated in an open field test. After, immunoreactivity to ΔFosB transcription factor, was analyzed in several brain areas. ΔFosB accumulates to high levels in brain after chronic stimulation and can be influenced by drugs exposure but also by natural rewarding stimuli. Finally, cathecolaminergic activity (ΔFosB/tyrosine-hydroxilase) was measured in ventral tegmental area. Results indicate that all animals were sensitive to alcohol-induced motor stimulation. MP-animals showed tolerance to this effect since PD17. Nevertheless, BP animals did not show tolerance even after the four days of test. Moreover, the intensity of this alcohol effect was more robust in BP than MP infants. A greater ΔFosB/TH-ir was observed in VTA in all alcohol-treated animals. Of major interest, MP-infants showed greater ΔFosB-ir in basolateral and central (CEA) amygdala, and this effect was exacerbated by alcohol in CEA? MP-infants.In conclusion, BP-infants were more sensitive to alcohol-induced motor stimulation. On the other hand, MP-offspring showed greater activation in brain areas related to anxiety behaviors as BLA and CEA, and were more sensitive to alcohol effects in this last area. These results are in line to the previously reported anxious behavioral profile in MP-adolescents and highlighted the importance of social experiences during early development.