Differential gene expression in SuCMoV tolerant and susceptible sunflower lines

MAILO D, POVERENE M, GIOLITTI F., LENARDON S.

Proceedings of 17th Intern. Sunflower Conference

International Sunflower Association

Lugar: Cordoba, Spain; Año: 2008; p. 635 - 640

Sunflower chlorotic mottle virus (SuCMoV) is one of the most widely distributed viruses affecting sunflower (Helianthus annuus L.) in Argentina. Symptoms include yellow blotches, reduced and distorted leaves and plant stunting. SuCMoV-susceptible (20016) and tolerant (B-133) sunflower lines were mechanically inoculated with SuCMoV and total RNA was isolated from infected leaf tissue, amplified and hybridized to an Arabidopsis oligonucleotide-microarray containing 22,000 genes. The gene expression profile in inoculated plants from both lines was statistically different from non-inoculated leaf samples. Eighty-eight genes were differentially expressed in the tolerant sunflower line. (SuCMoV) is one of the most widely distributed viruses affecting sunflower (Helianthus annuus L.) in Argentina. Symptoms include yellow blotches, reduced and distorted leaves and plant stunting. SuCMoV-susceptible (20016) and tolerant (B-133) sunflower lines were mechanically inoculated with SuCMoV and total RNA was isolated from infected leaf tissue, amplified and hybridized to an Arabidopsis oligonucleotide-microarray containing 22,000 genes. The gene expression profile in inoculated plants from both lines was statistically different from non-inoculated leaf samples. Eighty-eight genes were differentially expressed in the tolerant sunflower line.