Influence of Light Stress on the Accumulation of Xanthophylls and Lipids in Haematococcus pluvialis CCALA 1081 Grown under Autotrophic or Mixotrophic Conditions

DORIA, ENRICO; POPOVICH, CECILIA A.; TEMPORITI, MARTA; LEONARDI PATRICIA INES; DAMIANI, MARIA CECILIA; NIELSEN, ERIK

Journal of Marine Biology and Aquaculture

OMMEGA PUBLISHERS

Año: 2018 vol. 4 p. 31 - 35

2381-0750

The influence of light stress andtrophic environmental conditions on the production of astaxanthin, otherxanthophylls and lipids from an Argentinian strain of Haematococcus pluvialis was investigated. Microalgaecultures were incubated for two weeks in autotrophic or mixotrophic conditions(with sodium acetate in the culture medium) and subjected to two differentlight stresses. HPLC analyses showed that: 1) regardless of the growthconditions, microalgal cells accumulated most of the astaxanthin (about 90%) inesterified form; 2) maximal increase of astaxanthin level was observed in the culturegrown in autotrophic conditions subjected to moderate light stress (90 μmol photons m-2 s-1), while the same light regime inmixotrophic conditions led to a lower increase (only 25.8 fold); 3) in the caseof high light stress (350 μmol photons m-2 s-1), the adaptive response of microalgae also led to asignificant increase in the amount of astaxanthin both in autotrophic (22 foldincrease) and in mixotrophic (16 fold increase) conditions; 4) an inversecorrelation (R2 = 0.977) exists between the amountof lipids and that of astaxanthin accumulated by Haematococcus grown under different growthconditions; 5) lutein was found to be the most accumulated pigment inmicroalgae not subjected to light stress.