Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl

VAZQUEZ MB; BARRERA VIVIANA; BIANCHINOTTI MV

CANADIAN JOURNAL OF BOTANY

NRC Research Press

Lugar: Otawa; Año: 2015 vol. 93 p. 793 - 800

0008-4026

Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cerealcrops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon andenergy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using geneticmarkers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry.Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains andthe resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was testedwith a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient intransforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the bestperformance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studiesregarding treatment or amelioration of MM contaminated soils.