Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon.

DANIELA SORESI; ALICIA CARRERA; VIVIANA ECHENIQUE; INGRID GARBUS

MICROBIOLOGICAL RESEARCH

ELSEVIER GMBH

Año: 2015 vol. 177 p. 53 - 66

0944-5013

The wheat recombinant chromosome inbred line LDN(Dic-3A)10, obtained throughintrogression of a T. dicoccoides disomic chromosome 3A fragment into T. turgidum spp.durum var. Langdon, is resistant to fusarium head blight (FHB) caused by Fusariumgraminearum. To identify genes involved in FHB resistance, we used a cDNA-AFLPapproach to compare gene expression between LDN(Dic-3A)10 and the susceptibleparental line LDN at different time points post-inoculation. In total, 85 out of the ~500transcript-derived fragments (TDFs) were found to be differentially expressed: 36% and19% were upregulated in LDN(Dic-3A)10 and LDN, respectively, whereas 45% wereinduced in both genotypes. Several of the cloned TDFs showed similarity to proteinsinvolved in specific recognition of plant pathogens or associated with early responses toinfection. However, there was a group of TDFs that, in spite of being specific to theinoculation response did not show similarity to characterized proteins. The availability of T.aestivum genome sequences allowed in silico mapping of 28 TDFs and the identification ofseveral genes and regulatory regions. Analysis of promoter regions revealed the potentialexistence of shared transcription regulation mechanisms. For instance, a number of TDFassociated genes contained binding sites for WRKY transcription factors, which have been implicated in the regulation of genes associated with pathogen defense. Collectively, our results revealed specific pathogen recognition in the interactions of LDN and LDN(Dic-3A)10 with F. graminearum at the time that demonstrated changes in the expression ofseveral transcripts attributable to the presence of the QTL Qfhs.ndsu-3AS.