INIBIBB   05455
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BAHIA BLANCA
Unidad Ejecutora - UE
artículos
Título:
Changes in lipids containing long-and very long-chain polyunsaturated fatty acids in cryptorchid rat testes
Autor/es:
FURLAND, N. E.; MALDONADO, E. N.; AYUZA-ARESTI, P. L; AVELDAÑO, M. I.
Revista:
BIOLOGY OF REPRODUCTION
Editorial:
Society for the study of reproduction, Inc.
Referencias:
Año: 2007 vol. 77 p. 181 - 188
ISSN:
0006-3363
Resumen:
: Biol Reprod. 2007 Jul;77(1):181-8. Epub 2007 Apr 11.
CHANGES IN LIPIDS CONTAINING LONG- AND VERY LONG-CHAIN POLYUNSATURATED FATTY ACIDS IN CRYPTORCHID RAT TESTES.
Furland NE, Maldonado EN, Aresti PA, Aveldaño MI.
The aim of the present study was to examine the effects of experimental
cryptorchidism on rat testicular phospholipids and neutral lipids that contain
long-chain (C(18)-C(22)) and very long-chain (VLC) (C(24)-C(32)) polyunsaturated
fatty acids (PUFA). The weight of the cryptorchid testis was nearly half that of
the contralateral control at postsurgical Days 7-10 owing to the depletion of
germ cells. Concomitantly, the amounts of major glycerophospholipids (GPL) and
sphingomyelin (SM) per testis decreased. Both these lipids lost their
characteristic long-chain and very long-chain PUFA, notably 22:5n-6 and 28:4n-6,
respectively, which suggests that these species are linked to the membranes of
germ cells. In contrast, the amounts and concentrations of triglycerides (TG;
triacylglycerols and 1-O-alkyl-2,3-diacylglycerols) and cholesterol esters (CE)
increased several fold in the surviving cells (mainly Sertoli cells) in the
cryptorchid testis. All these neutral lipids, but especially CE, accumulated
large amounts of the major PUFA of the testis, 22:5n-6, as well as pentaenes with
longer carbon chains (i.e., 24:5n-6 in TG and 28:5n-6 in CE). This accretion
suggests that neutral lipids may store preformed PUFA coming from dying germ cell
GPL and also VLCPUFA no longer needed as a source of PUFA destined to assemble
new germ cell GPL. The lipid adjustments observed in cryptorchidism suggest a
possible role for Sertoli cell CE in the turnover and conservation of PUFA within
seminiferous tubules.