CONICET | Buscador de Institutos y Recursos Humanos

Canker control by the siderophore Pyochelin from Pseudomonas fluorescens. Adler Conrado1; Lami María Jesus1; de Cristóbal Ricardo1; Filippone María Paula2; Castagnaro Atilio Pedro2; Vincent Paula Andrea 1 1Instituto Superior de Investigaciones Biológicas (INSIBIO- CONICET-UNT). Tucumán, Argentina. 2Estación Experimental Agroindustrial Obispo Colombres (EEAOC) Tucumán, Argentina. This work shows the identification of a compound able to inhibit Xanthomonas citri pv. citri growth in vitro and in vivo. Firstly, we isolated from environmental samples, bacteria able to inhibit X. citri pv. citri (citrus canker agent) in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to pyochelin (Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of pyochelin, we evaluated the ability of pyochelin to generate reactive oxygen species (ROS) within a culture of X. citri pv. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of Pch in a model of canker disease. Pch showed to reduce canker formation on leaves of Eureka and Lisbon variety plants. These results show Pch as a promising compound for citrus canker biocontrol.