INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Biological activities of Yacon leaves in diabetic animals
Lugar:
Puerto Madryn
Reunión:
Congreso; XLVI REUNION ANUAL- SOCIEDAD ARGENTINA DE INVESTIGACION EN BIOQUIMICA Y BIOLOGIA MOLECULAR.; 2010
Resumen:
BIOLOGICAL ACTIVITIES OF YACON
LEAVES IN DIABETIC ANIMALS
Serra Barcellona C, Cabrera
WM, Genta SB, Sánchez SS.
INSIBIO (CONICET-UNT).
Chacabuco 461. T4000ILI SM de Tucumán, Argentina. E-mail:
cserrabarcellona@fbqf.unt.edu.ar
Medicinal plants have long
been an excellent source of pharmaceutical agents. Accordingly, the objectives
of many investigations are to discover and isolate new therapeutic agents based
on plant-derived compounds. Smallanthus
sonchifolius (yacon) is an Andean crop used for centuries by prehispanic
population in traditional medicine.
We demonstrated that a 10%
yacón leaves decoction (140mg dry extract/kg bw) produced a fast and
significant decrease in plasma glucose levels of diabetic rats.
The bioactivity screening of
organic extracts of yacón leaves revealed that butanol and ethyl acetate are
the most effective fractions to reduce postprandial glucose levels.
Phytochemical analysis of butanol extract showed the presence of caffeic,
chlorogenic and dicaffeoilquinic. Enhydrin was the major sesquiterpene lactone in
the ethyl acetate fraction and had significant hypoglycemic action at dose of 0,8mg
dry extract/kg bw.
In order to clarify the
mechanism of hypogycemic action of enhydrin and yacón decoction we tested the inhibitory activity
on α-glucosidase in
vitro and in vivo. The results showed that both samples
suppressed the carbohydrate absorption from intestine reducing the postprandial
increase of blood glucose.
Toxicity study of yacon decoction and
enhydrina using
normal Wistar rats demonstrated that yacon decoction and
enhydrin, are safe in the therapeutic dosage range.