HISTOPATHOLOGY OF THE RESISTANCE TO Colletotrichum gloeosporioides OF STRAWBERRIES WILD SPECIES RELATED TO COMMERCIAL STRAWBERRY

E. M. PARDO; C. F. GRELLET; S. M. SALAZAR; A. P. CASTAGNARO; J. C. DÍAZ RICCI; M. E. ARIAS

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Göttingen, Alemania; Año: 2010

0931-1785

With the aim to assess genetic resources exhibiting resistance to anthracnose, species of wild strawberries and related species were tested against virulent isolate of C. gloeosporioides L9. Results showed that the species Fragaria vesca L., Fragaria virginiana Duchesne and Fragaria chiloensis Duch. are susceptible; whereas species Duchesnea indica (Andrews) Focke, Duchesnea chrysantha (Zoll & Moritzi) Miq. and Potentilla tucumanensis Castagnaro & Arias showed higher levels of resistance. Histopathology revealed a differential response among susceptible and tolerant species. Significant thickening of the cell wall of epidermal cells, changes of stomata and mesophyll cells, presence of idioblastic cells with unknown content and the accumulation of large starch grains in atypical tissues, namely: stomata and parenchyma cells, were observed in species of the genus Duchesnea. But no morphological alteration was observed in Fragaria species as well as in P. tucumanensis that not showed apparently interaction with the isolate L9 of C.gloeosporioides. Oxidative burst was evaluated in leaf tissues with the fluorescent probe DCFH DA (2’,7’-Dichlorofluorescein diacetate) in susceptible and tolerant genotypes. Results showed that tolerant species present a notorious accumulation of H2O2 whereas susceptible species did not. We suggest that the changes observed are correlated with the resistance to disease.