LACTIC ACID BACTERIA AND BIFIDOBACTERIA FROM BULLFROG HATCHERY OF CORDOBA: EVALUATION OF ITS BENEFICIAL CHARACTERISTICS.

G. MONTEL MENDOZA; C.E. ALE; S.E. PASTERIS; M.C. OTERO; M.I. BÜHLER; M.E. NADER-MACÍAS

BIOCELL

INST HISTOL EMBRIOL-CONICET

Año: 2009

0327-9545

Aquaculture includes the frog culture, being Rana catesbeiana the most appropriate specie. In this area, diseases such as red leg syndrome (RLS) contributes to animal mass mortality and therefore to a high impact in the production costs. The use of probiotics is becoming increasingly popular in aquaculture, being Lactic Acid Bacteria (LAB) the most suitable microorganisms for this purpose. The aim of this study was to evaluate the beneficial properties (production of antagonistic compounds and surface properties) of 52 LAB and 5 Bifidobacterium spp. isolated from a hatchery of Córdoba and their differences with the LAB isolated from a hatchery of NOA. Results showed that strains inhibited at least one of the pathogens assayed by organic acid production (agar well diffusion method). A few strains (16) were found to produce hydrogen peroxide by using a specific method (MRS+TMB+peroxidase). Only one strain produced a bacteriocin active against S. epidermidis and L. monocytogenes. Surface properties assays showed that most of the strains were hydrophilic. However, 8 strains had a high hydrophobicity while 5 out of the 52 were autoagregantes. All Bifidobacterium strains were found to have a hydrophilic surface and only one showed antagonist activity. The LAB from hatchery of NOA has similar inhibitory profile and surface properties than those from Córdoba, however the percentage of H2O2-producing strains was higher. Results allow us to select potencial raniculture probiotic microorganisms to be used in different areas according to the biodiversity found.