Role lipids and genomic trascription in oocyte testosterone induced maturation

ARIAS TORRES AJ., PAEZ JB., AJMAT MT., BUHLER MI. Y ZELARAY¨¢N LI.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 67 - 67

0327-9545

ROLE OF LIPIDS AND GENOMIC TRANSCRIPTION IN TESTOSTERONE-INDUCED OOCYTE MATURATION Arias Torres AJ, P¨¢ez JB, Ajmat MT, B¨¹hler MI, Zelaray¨¢n LI. INSIBIO-UNT.Tucum¨¢n. E-mail: anajoarias@hotmail.com Oocyte maturation is a progesterone-mediated process that occurs in the absence of genomic transcription. Although progesterone (P4) is a potent promoter of R.arenarum oocyte maturation in vitro, little is known about other steroids in this process. In this study we analyzed the role of lipids and genomic transcription in the testosterone (T) mechanism of action during oocyte maturation. Oocyte maturation was induced by adding T (10-6 M) and time¡ª response curves were performed with actinomycin D (2mM), quinacrine (0-20¦ÌM) (PLA2 inhibitor) and indometacin (0-50¦ÌM) (COX inhibitor). In order to analyze the participation of phospholipid hydrolysis during maturation we used neomycin (0-2 mM). P4 (10-6 M) as control. Meiosis resumption was scored by germinal vesicle breakdown (GVBD) at 22 h. Our results showed that in R.arenarum oocytes T is a steroid inductor as effi cient as P4. Maturation is induced by T-independent genomic transcription (95¡À3 %GVBD). Inhibition of formation of AA by quinacrine does not affect oocyte T-induced maturation. COX inactivation by indometacin had an inhibitory effect on oocyte and follicle maturation. T-induced maturation was inhibited by neomycin in a dose dependent manner. In R. arenarum T induces maturation in a genomic transcription independent manner in which the second messenger derived from membrane phospholipids would be involved.