Expression of DNA Methyl Transferase Genes in Four-Cell Bovine Embryos Cultured in the Presence of Oviducal Fluid

BARRERA A.D.; GARCIA E.V.; SINOWATZ F.; PALMA G; MICELI, D C

ANATOMIA HISTOLOGIA EMBRYOLOGIA

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 p. 1 - 4

1439-0264

The possible epigenomic effect of oviductal fluid on expression of DNA meth- yltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cul- tured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviduc- tal fluid. These results may help reveal the role of oviductal factors in the regu- lation of DNMT expression. DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviduc- tal fluid. These results may help reveal the role of oviductal factors in the regu- lation of DNMT expression. in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviduc- tal fluid. These results may help reveal the role of oviductal factors in the regu- lation of DNMT expression.