Rat lefty/ebaf gene: isolation and expression analysis in the oviduct during early pregnancy stage

ARGAÑARAZ MARTÍN; VALDECANTOS PABLO; ABATE CARLOS; MICELI DORA C.

Genes and genetics systems

Genetics Society of Japan

Lugar: Japón; Año: 2006 vol. #MS6

1341-7568

Rat Lefty2/EBAF Gene:Isolation and Expression Analysis in the Oviduct During the Early Pregnancy Stage Argañaraz,Martin E.1 ,Valdecantos,Pablo A.1 ,Abate,Carlos M.2 and Miceli Dora C.1 * 1 Instituto Superior de Investigaciones Biológicas.INSIBIO (CONICET-Universidad Nacional de Tucumán),Chacabuco 461,4000.S.M.de Tucumán,Argentina 2 Planta Piloto de Procesos Industriales Microbiológicos.PROIMI (CONICET),Av. Belgrano y Pje.Caseros,4000.S.M.de Tucumán,Argentina (Received 3 November 2006,accepted 26 February 2007) It has been demonstrated,by RNA Arbitrarily Primed Polymerase Chain Reac- tion (RAP-PCR),that the endometrial bleeding associated factor (ebaf or lefty2 ) was expressed in rat oviduct.In this work we isolated and sequenced the full- length left2 cDNA from Rattus norvegicus oviducts and described its expression level in this organ during the estrous cycle and early pregnancy stage.The cod- ing deduced sequence (CDS)codifies a 40.91 kDa protein with a highly conserved TGF-â functional domain.RT-PCR semiquantitative analyses indicated that ovi- duct cells transcribe lefty2 among different stages of the estrous cycle with the maximum expression at diestrus phase.The highest expression of lefty2 was at the 4 th day after mating (five folds respect to day one),just when the embryos have completed their transit through the oviduct.The lefty2 expression declined rap- idly thereafter and the levels of their transcripts in the oviduct remained low until 7 th days after mating.