An automatic wrapped phase gradient-based method for spherical aberration correction in Digital Holographic Microscopy Un método automático basado en el gradiente de la fase envuelta para la corrección de la aberración esférica de fase en microscopía holográfica digital

ROMERO, G.G.; MONALDI, A.C.; CABRERA, C.M.; BLANC, A.V.

Optica Pura y Aplicada

Sociedad Espanola de Optica

Lugar: Madrid; Año: 2018 vol. 51 p. 1 - 8

0030-3917

Most digital holographic microscopy architectures introduce a microscope objective in the object arm of the micro-interferometer in order to increase lateral resolution but, as a consequence, a spherical phase aberration arises. The phase distortion must be corrected to achieve reliable phase information linked to the microscopic object under study. In this work, we present a fast and simple numerical method for automatically compensate the phase curvature from the wrapped phase gradient of the reconstructed hologram. Since non-unwrapping methods are required, computational cost is significantly reduced. Furthermore, no additional holograms recording is needed and it does not require prior knowledge of the object or the setup. The method is experimentally demonstrated by a phase contrast imaging of a Pediastrum cell.