A molecular recombination map of Antirrhinum majus

ZSUZSANNA SCHWARZ-SOMMER; THOMAS GÜBITZ; JULIA WEISS; PERLA GÓMEZ-DI-MARCO; LUCIANA DELGADO; ANDREW HUDSON; MARCOS EGEA-CORTINES

BMC PLANT BIOLOGY

BIOMED CENTRAL LTD

Lugar: Londres; Año: 2010 vol. 10 p. 275 - 285

1471-2229

AbstractBackground: Genetic recombination maps provide important frameworks for comparative genomics, identifyinggene functions, assembling genome sequences and for breeding. The molecular recombination map currentlyavailable for the model eudicot Antirrhinum majus is the result of a cross with Antirrhinum molle, limiting itsusefulness within A. majus.Results: We created a molecular linkage map of A. majus based on segregation of markers in the F2 population oftwo inbred lab strains of A. majus. The resulting map consisted of over 300 markers in eight linkage groups, whichcould be aligned with a classical recombination map and the A. majus karyotype. The distribution of recombinationfrequencies and distorted transmission of parental alleles differed from those of a previous inter-species hybrid. Thedifferences varied in magnitude and direction between chromosomes, suggesting that they had multiple causes.The map, which covered an estimated of 95% of the genome with an average interval of 2 cM, was used toanalyze the distribution of a newly discovered family of MITE transposons and tested for its utility in positioningseven mutations that affect aspects of plant size.Conclusions: The current map has an estimated interval of 1.28 Mb between markers. It shows a lower level oftransmission ratio distortion and a longer length than the previous inter-species map, making it potentially moreuseful. The molecular recombination map further indicates that the IDLE MITE transposons are distributedthroughout the genome and are relatively stable. The map proved effective in mapping classical morphologicalmutations of A. majus.