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MICROCHEMICAL CHARACTERIZATION OF BONE AROUND STRONTIUMDOPED BIOACTIVE GLASS PARTICLES Gorustovich Alejandro,1,2 Steimetz Tammy,3 Porto L¨®pez Jos¨¦ M2,4 1 Res Lab, Nat Atomic Energy Commission. CNEA, Salta 1 Res Lab, Nat Atomic Energy Commission. CNEA, Salta 1,2 Steimetz Tammy,3 Porto L¨®pez Jos¨¦ M2,4 1 Res Lab, Nat Atomic Energy Commission. CNEA, SaltaRes Lab, Nat Atomic Energy Commission. CNEA, Salta 2CONICETCONICET 3 Dept of Oral Pathol, School of Dentistry, University of Buenos AiresDept of Oral Pathol, School of Dentistry, University of Buenos Aires 4 Ceramics Div, Res Inst for Mater Sci and Technol, Mar del Plata The effects of strontium (Sr) on bone mineralization are controversial. The aim of the present study was to characterize the neoformed bone tissue around Sr-doped bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5% CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. The effects of strontium (Sr) on bone mineralization are controversial. The aim of the present study was to characterize the neoformed bone tissue around Sr-doped bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5% CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. Ceramics Div, Res Inst for Mater Sci and Technol, Mar del Plata The effects of strontium (Sr) on bone mineralization are controversial. The aim of the present study was to characterize the neoformed bone tissue around Sr-doped bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5% CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. 2, 24.5% CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. 2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt% SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout. Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15, 30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution, radiographed and processed for embedding in methyl-methacrylate resin.The characterization of undecalcified ground sections was performed using SEM-energydispersive X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone tissue was evaluated at each of the experimental times. The Ca:P ratio was determined. There was no bone Sr uptake by the neoformed bone tissue surrounding 45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group (1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively (p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles. tissue around 45S5.6Sr BG particles. p>0.05). No alterations in bone mineralization were observed in the neoformed bone tissue around 45S5.6Sr BG particles.