INTEMA   05428
INSTITUTO DE INVESTIGACIONES EN CIENCIA Y TECNOLOGIA DE MATERIALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Microchemical characterization of bone around strontium doped bioactive glass particles
Autor/es:
A.A. GORUSTOVICH; T. STEIMETZ; J.M PORTO L¨®PEZ
Lugar:
Buenos Aires
Reunión:
Jornada; XXIV Reuni¨®n anual de la Asociaci¨®n Argentina de Osteolog¨ªa y Metabolismo Mineral; 2007
Institución organizadora:
Asociaci¨®n Argentina de Osteolog¨ªa y Metabolismo Mineral
Resumen:
MICROCHEMICAL CHARACTERIZATION OF BONE AROUND STRONTIUMDOPED
BIOACTIVE GLASS PARTICLES
Gorustovich Alejandro,1,2 Steimetz Tammy,3 Porto L¨®pez Jos¨¦ M2,4
1 Res Lab, Nat Atomic Energy Commission. CNEA, Salta
1 Res Lab, Nat Atomic Energy Commission. CNEA, Salta
1,2 Steimetz Tammy,3 Porto L¨®pez Jos¨¦ M2,4
1 Res Lab, Nat Atomic Energy Commission. CNEA, SaltaRes Lab, Nat Atomic Energy Commission. CNEA, Salta
2CONICETCONICET
3 Dept of Oral Pathol, School of Dentistry, University of Buenos AiresDept of Oral Pathol, School of Dentistry, University of Buenos Aires
4 Ceramics Div, Res Inst for Mater Sci and Technol, Mar del Plata
The effects of strontium (Sr) on bone mineralization are controversial. The aim of the
present study was to characterize the neoformed bone tissue around Sr-doped
bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical
evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5%
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
The effects of strontium (Sr) on bone mineralization are controversial. The aim of the
present study was to characterize the neoformed bone tissue around Sr-doped
bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical
evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5%
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
Ceramics Div, Res Inst for Mater Sci and Technol, Mar del Plata
The effects of strontium (Sr) on bone mineralization are controversial. The aim of the
present study was to characterize the neoformed bone tissue around Sr-doped
bioactive glass particles (BG) implanted in rat tibia bone marrow by microchemical
evaluation. Melt-derived BGs were prepared from a base 45S5 BG (45% SiO2, 24.5%
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
2, 24.5%
CaO, 24.5% Na2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
2O,6% P2O5 in wt%). Sr-doped glass was prepared substituting 6 wt%
SrO for the CaO in 45S5 BG (45S5.6Sr). Thirty Wistar rats were used throughout.
Under anesthesia, 45S5 BG particles (300-350 ¦Ìm) were placed inside the medullary
compartment of the tibia (control), while 45S5.6Sr BG particles were implanted in the
contralateral tibia (experimental). The animals were sacrificed in groups of ten, at 15,
30, and 60 days post-implantation. The tibiae were resected, fixed in formalin solution,
radiographed and processed for embedding in methyl-methacrylate resin.The
characterization of undecalcified ground sections was performed using SEM-energydispersive
X-ray analysis (EDX).The presence of Sr, Ca, and P in the peri-implant bone
tissue was evaluated at each of the experimental times. The Ca:P ratio was
determined. There was no bone Sr uptake by the neoformed bone tissue surrounding
45S5.6Sr BG particles. The Ca:P ratio of bone tissue for the experimental group
(1.44¡À0.09,1.51¡À0.04,1.53¡À0.03) did not differ significantly from the values seen for the
control group(1.44¡À0.08,.51¡À0.06,1.53¡À0.04) at 15,30,and 60 days,respectively
(p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.
tissue around 45S5.6Sr BG particles.
p>0.05). No alterations in bone mineralization were observed in the neoformed bone
tissue around 45S5.6Sr BG particles.