Role of lipovitellins in the embryo development of the spider Schizocosa malitiosa.

CUNNINGHAM MÓNICA; LAINO ALDANA; COSTA FERNANDO; ROMERO SOFIA; NIETO ESTEBAN; GARCIA FERNANDO

Mar del Plata

Congreso; LI Reunión anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular.; 2015

Sociedad Argentina de Bioquímica y Biología molecular

In oviparous species, the only source of nutrients is the egg vitellus. In arthropods vitellus, it is usual to find carbohydrates, protein, and lipid which are being consumed throughout the development. These macromolecules can be found freely or as lipovitellins. In the present work, we studied the contribution of the lipovitellins as energetic resource and their consumption throughout the three stages of development utilizing the spider Schizocosa malitiosa as a model of study. From this analysis we could observe that the proteins of the lipovitellins remained constant throughout the three stages, representing in all the cases 20% of the proteins of the total homogenate of eggs. With respect to the lipid content it was observed that in early stages of development the lipids associated to lipovitellins represent approximately 25% of the lipids of total homogenate, whereas in advanced stages only 3% of the lipids of total homogenate correspond to lipovitellins. This decrease is mainly due to the consumption of TAG and PL that diminished 90% between 5 and 25 days of development, possibly to be used as energy source and for membrane formation of the embryo under formation. These basic results are necessary in order to understand the structure/function relationship of the macromolecules involved in the reproduction and development of such a numerous Order as that of Araneae.