INIBIOLP   05426
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE LA PLATA "PROF. DR. RODOLFO R. BRENNER"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Changes in Liver Fatty Acid Binding Protein (LFABP) expression modify lipid metabolism and cell biology of the enterocyte.
Autor/es:
RODRIGUEZ SAWICKI L; CÓRSICO, B
Lugar:
Varsovia
Reunión:
Congreso; International Conference on the Bioscience of Lipids (ICBL); 2011
Institución organizadora:
ICBL
Resumen:
Long chain fatty acids (LCFA) are needed as a source
of metabolic energy, membrane biogenesis, second messengers, modulation of gene
expression, and are associated with cell growth, differentiation, apoptosis and
inflammatory processes. Lipid hydrolysis in the intestinal lumen
releases great quantities of LCFA. Once inside the enterocyte, LCFA can be reversibly
bound to two homologous proteins: Intestinal and Liver Fatty Acid Binding
Proteins (I- and LFABP). Classical
functions proposed are as cytosolic buffers and transporters of hydrophobic
ligands, but new studies position them as key components for regulatory systems.
The present work was conducted to evaluate FABP´s specific roles in the lipid metabolism
and cell biology of the enterocyte.
For this purpose, we obtained a cellular model of Caco-2 cells with
ablated expression of LFABP and analyzed the assimilation and metabolism of
LCFA. Knock-down clones showed
marked variations in LCFA assimilation rates and differences in oleate
distribution among complex lipid species. Cell proliferation and
differentiation were also slowed in the clones. In addition, the effect of LCFA in the induction of an
inflammatory response in Caco-2 cells was studied, cytokines mRNA levels were
increased after LCFA exposure. These results indicate that LFABP plays a specific
role in the enterocyte lipid metabolism. Future studies will contribute to
asses whether intestinal FABPs are also involved in inflammatory processes
triggered by LCFA.