Synergistic effects of monoterpenes on Hep G2 and A549 tumor cells.

RODENAK KLADNIEW B; MANASSERO C; POLO M; GARCÍA DE BRAVO M

Puerto Madryn, Chubut, Argentina

Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

High intake of fruits and vegetables correlates epidemiologically with a reduction in cancer incidence. Among their components, the isoprenoids are phytochemicals that could have antitumor activity because of their multiple effects on mevalonate pathway. The aim of this work was to study the effect of linalool (L), cineole (C) or their combination on cell proliferation (CP) and lipid content in HepG2 and A549 human tumor cells. Cells were treated with individual monoterpenes (0-10000 µM) for 48 h and their IC50 (dose resulting in 50% cell growth inhibition) were determined. Synergism between both monoterpenes was evaluated using half of their IC50 values. Cellular proliferation, cholesterol (Cho), triglyceride (TAG) and phosphorus (P) content were determined. In HepG2 cells, L and C individually produced 11 and 9% inhibition of CP respectively, without altering the lipid content, while their combination decreased CP (40%), content of Cho (20%) and TAG (36%). In A549 cells, L and C inhibited CP by 10 and 15% respectively, resulting their combination in a decrease in CP (59%). In conclusion, according to our results, L and C have synergistic effects on the inhibition of CP and diminution of Cho and TAG in human tumor cells.