INIFTA   05425
INSTITUTO DE INVESTIGACIONES FISICO-QUIMICAS TEORICAS Y APLICADAS
Unidad Ejecutora - UE
artículos
Título:
Characterization of poly-e-caprolactone/polyfumarate blends as scaffolds for bone tissue engineering
Autor/es:
JUAN MANUEL FERNANDEZ; M. SILVINA MOLINUEVO; ANA M. CORTIZO; ANTONIO D. MCCARTHY; M. SUSANA CORTIZO
Revista:
JOURNAL OF BIOMATERIALS SCIENCE - POLYMER EDITION
Editorial:
VSP B.V.
Referencias:
Lugar: Netherlands; Año: 2009
ISSN:
0920-5063
Resumen:
There is considerable interest in the design of polymeric biomaterials that can be used for the repair of bone
defects. In this study, we used ultrasound to prepare a compatibilized blend of poly(å-caprolactone) (PCL)
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
å-caprolactone) (PCL)
and poly(diisopropyl fumarate) (PDIPF). The formation of post-sonication inter-polymer coupling products
was verified by SEC analysis of a blend with azo-labeled PDIPF. We also analyzed the physicochemical
and mechanical properties of the compatibilized blend. When compared to PCL alone, the PCL/PDIPF
blend showed no difference in its resistance as evaluated by the elastic modulus, although it did show a
50% decrease in ultimate tensile stress (P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.
P <0.05) and an 84% decrease in elongation-at-break (P <0.05).
However, the mechanical properties of this blend were comparable to those of trabecular bone. We next
evaluated biocompatibility of the PCL/PDIPF blend, and of homo-polymeric PCL and PDIPF films for
comparison, with UMR106 andMC3T3E1 osteoblastic cells. Osteoblasts plated on the compatibilized blend
adhered and proliferated more than on either homo-polymer, showed a greater number of cellular processes
with a better organized actin cytoskeleton and expressed more type-I collagen and mineral, both markers
of osteoblast phenotype. These results support the hypothesis that this new compatibilized blend could be
useful in future applications for bone regeneration.