An updated of Entomophthoromycota fungi pathogens of insects fromArgentina

CLAUDIA CRISTINA LOPEZ LASTRA; MANFRINO, ROMINA G.; GUTIERREZ, A.C.,.,; ANDREA V. TOLEDO; JOERGEN EILEMBERG; ANNETTE B. JENSEN

Lima

Congreso; IX Congreso latinoamericano de micologia; 2017

Asociacion latinoamericana de micologia ALM

An updated of Entomophthoromycota fungi pathogens of insects from ArgentinaLópez- Lastra, Claudia C., 1,1 Manfrino Romina G. 1,2, Gutierrez, Alejandra C. 1,3, Toledo, Andrea V.2,4, Eilenberg, Joergen 3,5and Jensen Annette B. 3,6Entomophthoralean fungi have been scarcely studied in Argentina, and so far only 16 out of 240 known species of entomophthorales have been recorded in agricultural pests like aphids, or sanitary insect vectors as houseflies. The objective of this study was to update the knowledge of entomophthoralean species diversity and their geographical distribution in Argentina. Infected insects and mite pests were surveyed from horticultural, soybean and cereal crops from Buenos Aires, Corrientes, Entre Ríos and Santa Fe provinces. Fungal characterization was made using morphological features such as nuclei number per conidium, diameters of conidia and nuclei, and presence/absence of rhizoids, cystidia and resting spores. Also, we realized their molecular characterization of rDNA 28 S, 18 S, and ITS, (ITS, SSU, LSU). A few of the species were isolated in pure cultures and deposited at culture collections. We documented seven new host-pathogen relationships including the fungi Conidiobolus sp., Entomophthora sp. and Zoophthora radicans, who were found infecting insect hosts belonging to the orders Diptera, Hemiptera, Lepidoptera and Thysanoptera from Pampasic and Litoral regions of Argentina. These new records did not only increase the host range of entomophthoralean fungi, but also they gave new insights about the geographical distribution of theses fungi.Key words: entomopathogenic, Entomophthorales, diversity1 Laboratorio de hongos entomopatogenos, CEPAVE Centro de estudios parasitológicos y de vectores (CONICET-UNLP); Boulevard 120 e/ 61 y 62 s/n (1900) La Plata;Argentina.1 claudia@cepave.edu.ar2 Laboratorio de hongos entomopatógenos, CEPAVE Centro de estudios parasitológicos y de vectores (CONICET-UNLP); Boulevard 120 e/ 61 y 62 s/n (1900) La Plata; Argentina2 romimanfrinohotmail.com1Laboratorio de hongos entomopatógenos, CEPAVE Centro de estudios parasitológicos y de vectores (CONICET-UNLP), Boulevard 120 e/ 61 y 62 s/n (1900) La Plata; Argentina3 gutialeja@gmail.com2CINDEFI Facultad de Ciencias Agrarias y Forestales UNLP, calle 60 y 118; (1900) La Plata; Argentina4 andytoledo75@yahoo.com.ar3 Department of Plant and Environmental Sciences; University of Copenhagen; Frederiksberg; Denmark5 jei@plen.ku.dk3Department of Plant and Environmental Sciences; University of Copenhagen, Frederiksberg; Denmark6 abj@plen.ku.dk1