INFIVE   05416
INSTITUTO DE FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Genetic variabilityr in natural populations of Paspalum dilatatum Poir analyzed by means of morphological traits and molecular markers
Autor/es:
MARIA VICTORIA GARCIA, PEDRO A. BALATTI AND MIGUEL J. ARTURI
Revista:
GENETIC RESOURCES AND CROP EVOLUTION
Referencias:
Año: 2007 vol. 54 p. 935 - 946
ISSN:
0925-9864
Resumen:
Native species show adaptive traits
that are difficult to find in introduced species. The
Pampas region in Argentina is a valuable nature
reserve of grasses and Paspalum dilatatum Poir. is
one of the most important grasses found there.
Based on ploidy level and on morphological
traits, five biotypes of P. dilatatum have been
described. Two of them were included in this
study: a tetraploid biotype with sexual reproduction
and a pentaploid biotype with apomictic
reproduction. We analyzed the genetic diversity
in eight native populations from the Salado basin,
Argentina, using both quantitative traits and
molecular data (RAPD) with these aims: to obtain
information of the degree of phenotypic
variation in that area, to know which the pattern
of distribution of this variation is and to look for
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.Paspalum dilatatum Poir. is
one of the most important grasses found there.
Based on ploidy level and on morphological
traits, five biotypes of P. dilatatum have been
described. Two of them were included in this
study: a tetraploid biotype with sexual reproduction
and a pentaploid biotype with apomictic
reproduction. We analyzed the genetic diversity
in eight native populations from the Salado basin,
Argentina, using both quantitative traits and
molecular data (RAPD) with these aims: to obtain
information of the degree of phenotypic
variation in that area, to know which the pattern
of distribution of this variation is and to look for
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.P. dilatatum have been
described. Two of them were included in this
study: a tetraploid biotype with sexual reproduction
and a pentaploid biotype with apomictic
reproduction. We analyzed the genetic diversity
in eight native populations from the Salado basin,
Argentina, using both quantitative traits and
molecular data (RAPD) with these aims: to obtain
information of the degree of phenotypic
variation in that area, to know which the pattern
of distribution of this variation is and to look for
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.
association between molecular markers with
populational or biotypic differentiation. Cluster
analysis based on morphological data grouped the
individuals of the different populations by ploidy
level. Molecular markers showed the inverse
situation because individuals were grouped by
geographic origin as opposed to biotype. Moreover,
since RAPD did not discriminate between
biotypes with sexual or apomictic reproduction,
they are probably not associated with mating
system. The results let us conclude that polygenic
traits such as LP, LBSR, NRT and NSP can discriminate
between biotypes and molecular
markers such as bands 12, 40, 19 and 46 can be
used to discriminate among populations, probably
because they detect neutral variation.