Differential regulation of laccase gene expression in Coriolopsis rigida LPSC No. 232

MARIO CARLOS NAZARENO SAPARRAT; BALATTI, P. A.; MARTÍNEZ, M. J.; JURADO, M.

Fungal Biology

Elsevier Ltd.

Año: 2010 vol. 114 p. 999 - 1006

1878-6146

Two laccase isoenzyme genes (lcc2 and lcc3) from the white-rot fungus Coriolopsis rigidawere cloned, and together with the previously described lcc1, their transcript levels wereanalysed by Quantitative RT-PCR in order to study their expression patterns under a rangeof putative inducers (Cu2+, Mn2+, Fe3+, 2,6-dimethoxy-1,4-benzoquinone, H2O2, caffeine,amphotericin B and syringic acid). The highest induction was observed for lcc1 in presenceof copper, and thus, a kinetic study was performed to analyze its effect on temporary lcc1gene expression. Our results showed that upregulation due to copper was linked to growthstage, being highest during the trophophase and decreasing during the idiophase. AmphotericinB increased levels of transcripts of lcc1 and lcc2, syringic acid upregulated lcc1 andlcc3 and 2,6-dimethoxy-1,4-benzoquinone induced lcc2 and lcc3. Possible reasons for whylaccase genes from C. rigida are differentially regulated at the transcriptional level arediscussed.