INHIBITION OF THE MUTAGENIC EFFECTS OF SULFATIAZOLE-NITRITE MIXTURE BY L-ASCORBIC ACID AND GREEN TEA

ANA PONTORIERO; MARCELA RIZZOTTO*

Facultad de Ciencias Químicas, Universidad Nacional de Córdoba

Congreso; Primera Reunión Internacional de Ciencias Farmacéuticas – RICiFa 2010; 2010

Universidad Nacional de Córdoba, Universidad Nacional de Rosario

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} p.MsoBodyText3, li.MsoBodyText3, div.MsoBodyText3 {margin:0cm; margin-bottom:.0001pt; text-align:center; mso-pagination:widow-orphan; mso-layout-grid-align:none; text-autospace:none; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US; mso-fareast-language:EN-US; font-weight:bold;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> INHIBITION OF THE MUTAGENIC EFFECTS OF  SULFATIAZOLE-NITRITE MIXTURE BY L-ASCORBIC ACID AND GREEN TEA        The concept of chemoprevention (use of natural or synthetic compounds to prevent cancers) has great appeal. N-nitroso compounds are mutagens which can be formed in vivo due to the reaction between amides and/or amines with nitrite. L-ascorbic acid (asc.) and green tea polyphenols can react with nitrite diminishing or removing the nitrosation risk. Sulfonamides, widely used by their properties, present potential risk of nitrosation in stomach because the presence of these functions.   In the present work we assay the chemopreventive action of  asc. and green tea as antimutagens (AM) on the mutagenicity of a reaction mixture formed by sodium sulfathiazole (NaST) and NaNO2 in acidic medium by mean of the Ames test. Total inhibition of the mutagenicity of the reaction mixture NaST-NaNO2 was achieved for equimolar quantity of L-ascorbic acid with nitrite. Only partial inhibition was achieved with diluted aqueous extract of green tea.