IFISE   05411
INSTITUTO DE FISIOLOGIA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Features of partitioning pattern of two pancreatic enzymatic precursors:
Autor/es:
PELLEGRINI, L.; PICÓ, G.; NERLI, B
Revista:
J. Chromatography B
Editorial:
Elsevier
Referencias:
Lugar: Dublin; Año: 2008 vol. 870 p. 1 - 7
Resumen:
The partitioning behaviour of bovine trypsinogen and alpha-chymotrypsinogen, enzymatic precursors
with similar physicochemical properties, was investigated in different polyethyleneglycol/sodium citrate
aqueous two-phase systems. The effect of different factors such as polyethyleneglycol molecular
weight, pH, tie line length and temperature was also examined. The increase of pH and the decrease of
polyethyleneglycol molecular weight displaced the partitioning equilibrium of both proteins to the top
phase. An enthalpyentropy compensation pattern was observed, indicating the participation of water
molecules in the partitioning mechanism. TRPz phase equilibrium showed to be more displaced to the
citrate-rich phase than ChTRPz for most of the assayed systems. From a practical view, the aqueous twophase
system formed by polyethylenglycol of molecular weight 1450 and sodium citrate pH 8.20 showed
the best capability for separating both proteins. When a mixture formed by equal quantities of both
zymogens was partitioned in this system, significant recoveries (about 60%) were obtained. Purity values
were improved significantly (8489%) by either developing a second extractive step or increasing the
topbottom volume ratio