IFISE 05411
INSTITUTO DE FISIOLOGIA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion
Autor/es:
ERICKSON B; SELVAN SP; KO KW; KELLY K; QUIROGA, ARIEL DARIO; LI, LENA; NELSON, RANDY; KING-JONES K; JACOBS RL; LEHNER, RICHARD
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2013 p. 1113 - 1123
ISSN:
1388-1981
Resumen:
Abstract
Hepatic
triacylglycerol levels are governed through synthesis, degradation and export
of this lipid. Here we demonstrate that enforced expression of hepatic lipase
in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a
significant decrease in the incorporation of fatty acids into cellular
triacylglycerol and cholesteryl ester accompanied by attenuation of secretion
of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion
of intracellular lipid storage increased the expression of peroxisome
proliferator-activated receptor á and its target genes and augmented oxidation
of fatty acids. These data show that 1) hepatic lipase is active in the
endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular
lipid metabolism and lipoprotein secretion
