Alterations in Aggregation kinetics of glycated Erythrocyte by in vitro Anesthesia

ANALÍA I. ALET; MARCUS VINICIUS BATISTA DA SILVA; BIBIANA RIQUELME; MARTÍN TODERI

Lima

Congreso; Biophysical Society Thematic Meetings - Revisiting the Central Dogma of Molecular Biology at the Single-Molecule Level; 2019

Biophysical Society

The erythrocyte membrane has negative electrical charges. When blood flow is slower or null, the erythrocytes form aggregates similar to stacks of coins called rouleaux. In some vascular diseases, such as diabetes, there are other types of globular aggregates called ?clusters?, which could be due in part to the reduction of surface charge by glucose effect. On the other hand, previous studies indicate that propofol could alter the hemorheological properties of the erythrocyte membrane, affecting principally diabetic patients. To evaluate this hemorheological effect of anesthesia, we studied the kinetics of aggregation of in vitro glycated blood samples by incubating them with different commonly used anesthetics (Propofol, Remifentanil and Vecuronium Bromide). For this purpose, red blood cells (RBCs) from healthy donors were preincubated with glucose solutions or phosphate saline solution for 5 hours. Subsequently, incubation was carried out with the different anesthetics and their combinations for 30 minutes (Propofol 4 μg/mL whole blood, Remifentanil 10 ng/mL plasma, Vecuronium bromide 0.15 mg/mL plasma). After washing, the RBCs were suspended in autologous plasma at 40%. To determine the aggregation kinetics of RBCs, the ?Agregámetro Eritrocitario de Chip Óptico? was used. This instrument was developed by the research group of Biomedical Physics of the Rosario Institute of Physics (CONICET-UNR) and is based on the laser transmission technique analyzing the intensity of light transmitted as a function of time. These data allow to obtain the sylectograms (intensity vs. time) to obtain the characteristic aggregation parameters for each sample. Results show that Aggregation Amplitude of glycated RBCs were altered in comparison with their respective control. Moreover, greater difference is observed in RBCs incubated with vecuronium bromide and the mixture of remifentanil and vecuronium bromide showing that they are aggregated more rapidly. In conclusion, anesthetics can affect the parameters of aggregation kinetics increase the speed of the aggregation process in glycated red blood cells.