Study of Red Blood Cell interaction by side scattering and forward scattering biospeckle

RIQUELME, BIBIANA DORIS; GALIZZI, GUSTAVO; TODERI, MARTIN

Lima

Conferencia; Latin America Optics and Photonics Conference 2018; 2018

The Optical Society (OSA)

The study of the erythrocyte, also called red blood cell (RBC), interaction has a wide spectrum of interest, fromresearch on blood viscoelastic characteristics [1, 2] to immediate application to the clinical environment [3]. Inparticular, the tendency of these investigations is to arrive to non-invasive techniques and to minimize the amountof sample needed to perform the study. In vivo RBC adhesion phenomena have a determinant role in blood flowand vascular resistance. It is of great importance to develop techniques and equipment which allow evaluation and characterization of the RBC interaction due to its implications in vascular pathologies such as diabetes and hypertension [4]. When a scattering medium is illuminated with a coherent light source, an interference pattern is observed at the image plane. The final optical field is presented as a distribution of speckles. This interferometricphenomenon carries information of the morphology of the illuminated sample. When the aforementioned distribution is time dependent, the phenomenon is called dynamic speckle. Moreover, if this variation in time is the consequence of a biological process, it is defined as biospeckle. In this sense, biospeckle Laser (BSL) has been observed as a sensitive way to record changes in biological samples and has been adopted as a reliable tool that can be applied in many areas, from agriculture [5] to medicine, particularly blood flow [6].