CONICET | Buscador de Institutos y Recursos Humanos

When blood is at rest, erythrocytes (RBC) aggregates forming rouleaux. However,in pathological conditions (diabetes) the RBCs anionic charge can be reduced byglycation, reducing cell repulsion and favoring RBC aggregation as well as changesin viscoelastic parameters. This effect can be increased in surgical procedureswhen the patient is under anesthetic drugs, such as propofol.Blood samples from healthy donors were collected by venipuncture in sterile vialscontaining EDTA and washed two times with PBS. The RBCs were glycated in vitrowith glucose solutions of 0.25%, 0,5% and 1% in PBS. After washed, the RBCs wereresuspended in autologous plasma (40%) and diluted with PBS+propofol 4 μg/mL(1:1) for propofol treatment (37ºC, 30 min).Viscoelastic parameters were studied using an Erythrocyte Rheometer: δ (thephase shift between deformation and applied shear stress), DI (ErythrocyteDeformability Index), μ (Elastic Modulus), η (Surface Viscosity of Membrane), G?(Dynamics Elasticity), G? (Dynamics Lost), η? (Viscous component of DynamicsViscosity), and η? (Elastic component of Dynamics Viscosity). For digital imageanalysis the isolated cell coefficient (CCA) was calculated.The samples glycated and treated with propofol were altered in comparison withthe ones only glycated. The DI increased with the glucose concentration. Theelastic modulus changed in a very small ration. The viscosity increasedcorrelatively with the glucose concentration. For the G?, G?,η? and η? parameters,the values did not show significant differences.The elastic modulus presented a tendency to decrease while increasing glucoseconcentration, perhaps causing hardening of the RBC cytoskeleton. When treatedwith propofol, the elastic modulus further diminished. Therefore, Propofol maycould further harden the cytoskeleton. This hardening may be retarding theresponse between the applied cutting tension and its deformation.