Evaluation of linear and nonlinear dynamic viscoelastic behavior of stored red blood cells

RIQUELME, BIBIANA DORIS; CASTELLINI, HORACIO; DANIELI, VIRGINIA; DI TULIO, LILIANA; RELANCIO, M. F.; FORESTO, PATRICIA GLADYS

Madrid, España

Congreso; XVIIth Regional Congress of the International Society of Blood Transfusion; 2007

ISBT

The linear viscoelastic behavior of the red blood cell membrane (RBC) was studied in previous works proposing an experimental method to determine their stationary and dynamic viscoelastic parameters based on laser diffractometry technique. In the present work the nonlinear component of dynamic viscoelasticity of the red blood cell membrane by nonlinear time series analysis is used for evaluating the alterations in the erythrocyte viscoelasticity during the storing time. For such aim, time series of test in vitro of stored red blood cell samples under oscillating shear stress was obtained. Packed RBC from whole blood of a healthy donor collected with CPDA-1 solution was stored under blood bank conditions during 45 days. Each week after donation a sample was taken from the concentrated RBC storage bag under sterilized conditions to be analyzed. The measurements were obtained using the Erythrodeformeter in oscillating regime at 0.5, 1 and 1.5 Hz. The photometrical reading was used to determine the linear and non-linear parameters. The signal filtrate was used for suppress any linear behavior as well as represented by a system of linear ordinary differential equations. The linear and non-linear parameter were presented and their sensibility for detect the viscoelastic erythrocyte alterations was analyzed. These results allow to extend the present knowledge on dynamics of the cellular membrane, which occurs at similar oscillating shear stress in the blood circulation, and also to evaluate the post-transfusional survival of stored red blood cells. Key Words: Nonlinear viscoelasticity, recurrence plots, erythrocyte membrane, dynamic viscoelasticity, Erythrodeformeter.