Permeability and other physicochemical properties. A comparative Study of Colagen Membranes

G. RUDERMAN; E.J. TOLOSA; I.G. MOGILNER; J.R. GRIGERA

Rosario

Congreso; XXXV Reunión Anual de la Sociedad Argentina de Biofísica; 2006

Sociedad Argentina de Biofísica

Collagen membranes of several thickness, with and without molecular order were prepared according to what is stated in our patent nº253249 of April 30th 1999. Permeability of these membranes for rifamycin, ascorbic acid and NaCL were measured at 37°C. These measurements were also performed to “Membracel” a membrane which can be bought at a chemist (with no molecular order, since the ordered ones do not exist yet for application purposes) and it is said to be of the same protein. Weight and volume of all these membranes were also measured. The results show that “Membracel” present a permeability of about one half of the value found to our non-ordered membranes of an equivalent thickness for NaCl and one-third for ascorbic acid. But, this difference is higher for rifamycin “Membracel” does not let the antibiotic to get through it a few minutes after started the transport process. (At the beginning of the process the permeability of “Membracel” was about ¼ of the same value obtained for our non ordered one). Similar results were found for the permeability of our ordered membranes when comparing with the industrial one. But, the ordered membranes present permeability slightly lower than the non-ordered ones. The measured density of “Membracel” was about three times that value for our non-ordered membrane of the same thickness. Comparing among our membranes, the ordered membranes present higher densities than the non-ordered ones, being higher as they were thinner. Also, preliminary studies of IR spectroscopy of the membranes were performed. The obtained spectra of all our membranes were similar, while the industrial one presents two extra peaks of non-identified compounds.