Synthesis of bacterial cellulose scaffolds with silver phosphate microparticles for skin wounds/burns treatment". Bernardo Bayon, Vera Alvarez, Guillermo Castro.

GUILLERMO CASTRO; VERA ALVAREZ; BERNARDO BAYÓN

Bariloche, Argentina

Congreso; XVII Encuentro de Superficies y Materiales Nanoestructurados ? NANO 2017 22, 23 y 24 de mayo de 2017 - Centro Atómico Bariloche - San Carlos de Bariloche - Argentina; 2017

Comisión Nacional de Energía Atómica

In-situ synthesis of silver phosphate (AgP) microparticles over the surface of bacterial cellulose (BC) provides scaffolds with broad-spectrum antimicrobial activity against both Escherichia coli and Staphylococcus aureus. Development of AgP microparticles over the surface of BC allowed encapsulating more than 5 timesciprofloxacin compared to naked BC. Addition of pectin during the AgP synthesis on BC surface increases 8% the ciprofloxacin loading and 40% the antibiotic release. The reuse AgP-BC ciprofloxacin loaded scaffolds in fresh cultures of both tested microbes showed high inhibition haloes without any signal of microbial resistance in 30 days. Additionally, microbial biofilms of E. coli and S. aureus were mostly killed by AgP-BC scaffolds loaded with ciprofloxacin in one hour. Biophysical characterization of the hybrid AgP-BC scaffolds were performed by scanning electron microscopy, vibrational spectroscopy, calorimetric techniques and X-ray diffraction displaying spheroid structure with high homogenous distribution of silver phosphate microparticles on BC scaffold surfaces. The properties of the novel AgP-BC are very promissory for the development of new therapeutic dressing patch for the prevention and treatment of microbial infections in skin wounds