Nanocomposite bacterial cellulose film containing doxorubicin loaded solid lipid nanoparticles for local antitumoral treatment

CACICEDO ML; ISLAN GA; DURAN N; CASTRO GR

Trivandrum

Conferencia; International Conference on New Horizons in Biotechnology (NHBT-2015); 2015

Abacterial cellulose (BC) biogel containing solid lipid nanoparticles (SLN) wasdeveloped as a local system for sustained release of doxorubicin (Dox). BC membraneswere synthesized in static cultures of Gluconacetobacterhansenii and purified for further uses. SLN composed of Crodamol? SS lipidwere dispersed into Pluronic F68 solution for 50 minutes under ultrasonication.Previously, Dox was completely dissolved into DMSO solution and mixed with thelipidic phase. After synthesis, the Dox encapsulation percentage into SLN was around92.9 ± 1.9 %. SLN-Dox released a 98% in buffer solution at pH 5.0 and a 65% atpH 7.4 at 37ºC after one week, which represents an interesting approach incancer treatment considering the slow acidic pH environment found aroundtumors. BC membranes of 6 mm in diameter were capable of incorporating SLN-Dox at10%, reaching therapeutic Dox concentrations and able to release thenanoparticles. Confocal fluorescent microscopy corroborated the incorporationof fluorescent nanoparticles into films. In addition, BC-SLN-Dox membranes wereobserved at SEM microscope and images revealed the presence of nanoparticlesfused to the cellulose fibers, indicating the presence of a new architecture ina hybrid hydrophilic/hydrophobic material. These results show that BC nanocompositeis a promising prototype for improve the local treatment of tumors.