Characterization of Polyvinyl alcohol Pectin cryogel for transdermal delivery

YANINA N MARTINEZ; JIMENA S GONZALEZ; VERA A ALVAREZ; GUILLERMO R. CASTRO

Bariloche

Encuentro; XIV Encuentro de Superficies y Materiales Nanoestructurados; 2014

Centro Atomico Bariloche

Polyvinyl alcohol (PVA) and PolyVinyl Alcohol- Pectin (PVA-P) lms containing the fluouroquinolone enrofloxacin, and an keratinase enzyme, which were developed to treat wounds and eschars, acting as a patch in the infected skin areas.In this work, characterization of the polymeric blend as well as PVA-P keratinase-enrofloxacin matrix was achieved to get insight on structure-function relationship. The presence of pectin in PVA cryogel in- crease the size of PVA nanodomains determined by X-ray diffraction (XRD) patterns which implies an interaction between both polymers. SAXS analysis showed changes in 0.84 nm of PVA nanodomains when enrofloxacin and keratinase were immobilized into PVA-P cryogels. These results conrfirmed the incorporation of both the enzyme and enrofloxacin in the polymeric matrix. A model involving the complex formation between the enzyme and enrofloxacin allocated through and between PVA nanodomains is proposed and it is well-correlated with results obtained in previous work in where enrofloxacin kinetic release is retarded by the presence of keratinase in the gels.