Evaluation of antioxidant activity of emulsan in vitro

CASTRO, GUILLERMO R; ZULUAGA NATALIA; PANILAITIS BRUCE,; KAPLAN DAVID L.

Biotechnology (Based on NHBT-2007)

Asiatech Publishers Inc

Lugar: New Dheli; Año: 2009; p. 13 - 22

The scavenging activities of emulsan against reactive oxygen and nitrogen species (ROS and RNS, respectively) were investigated in vitro using Rose Bengal as reporter. Emulsan, a biopolymer complex produced by Acintobacter venetianus strain RAG-1 was produced, purified and supplemented to Rose Bengal solutions containing ROS produced by the Fenton reaction or Tempon as an RNS model. The effect of pH, temperature and ionic strength was studied in both ROS and RNS systems. Emulsan was found active in scavenging ROS and RNS in a dose dependant manner. The results show that emulsan scavenging activity is consistent under broad range of experimental conditions.