Production of pectinases by A. niger: Influence of fermentation conditions

MARTOS, M.A.; MARTINEZ VAZQUEZ, F.; BENASSI, F.O.; HOURS, R.A.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

INST TECNOLOGIA PARANA

Lugar: Curitiba, Brazil; Año: 2009 vol. 52 p. 567 - 572

1516-8913

Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol.