Pectinolytic yeasts from cold environments: novel findings of Guehomyces pullulans, Cystofilobasidium infirmominiatum and Cryptococcus adeliensis producing pectinases

VERO, SILVANA; ALBANESI, AGUSTIN; GARMENDIA, GABRIELA; CAVELLO, IVANA; CAVALITTO, SEBASTIÁN FERNANDO; FRATEBIANCHI DE LA PARRA, DANTE

EXTREMOPHILES

SPRINGER TOKYO

Lugar: Tokyo; Año: 2017 vol. 21 p. 319 - 329

1431-0651

One hundred and three yeastsisolated from soil samples from King George Island and Tierra del Fuego provincewere screened in relation with their capability to produce pectinolyticenzymes. Although all the yeasts showed well-developed colonies at 20 °C, onlyeight showed a clear halo around the colony, indicative of pectin degradation.A secondary screening demonstrated that only four yeasts were capable toproduce pectinases at low temperatures (8 °C). It could be seen that theselected yeasts were able to grow and produce high levels of polygalacturonase activitywhen submerged fermentations were performed using pectin-containing fruitwastes as substrates. None of the strains produced neither lyase norrhamnogalacturonan hydrolase activities. Regarding pectin esterase activity, itwas only produced in lower amounts by G. pullulans 8E (0.022 U ml−1). A TLC analysis of the substrate cleavage patternof the pectinolytic systems was consistent with an endo-type activity. Theclarification of apple juice was only accomplished by G. pullulans pectinolyticsystem, with a clarification of 80% (%T650) using 4 U/ml of enzyme at 20 °C. Asfar as we concern this work describes for the first time the production ofpectinases by the cold adapted yeasts species Cystofilobasidium infirmominiatum,Cryptococcus adeliensis and G. pullulans