Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing

FESTA SABRINA; MORELLI IRMA S; MACCHI MARIANELA; COPPOTELLI BIBIANA M; CORTÈS FEDERICO

FEMS MICROBIOLOGY ECOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2016 vol. 92

0168-6496

The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil(PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications inbioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in responseto inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons.In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPKno improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observedthat inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore,independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting itmay use other resources to survive. On the other hand, the inoculation did not influence the bacterialcommunity of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase onActinomycetales and Sphingomonadales orders, while inoculation caused a relative decline ofActinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase ofSphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between thosegenera, our results suggest that this would not be directly related to PAH degradation