Pectinolytic yeasts from cold environments: novel findings of Guehomyces pullulans, Cystofilobasidium infirmominiatum and Cryptococcus adeliensis producing pectinases

CAVELLO, I; GARMENDIA, G; ALBANESI, A; VERO, S; FRATEBIANCHI, D; CAVALITTO, S. F

EXTREMOPHILES

SPRINGER TOKYO

Lugar: Tokyo; Año: 2016 vol. 21 p. 319 - 329

1431-0651

One hundred and three yeasts isolated from soilsamples from King George Island and Tierra del Fuegoprovince were screened in relation with their capabilityto produce pectinolytic enzymes. Although all the yeastsshowed well-developed colonies at 20 °C, only eightshowed a clear halo around the colony, indicative of pectindegradation. A secondary screening demonstrated that onlyfour yeasts were capable to produce pectinases at low temperatures(8 °C). It could be seen that the selected yeastswere able to grow and produce high levels of polygalacturonaseactivity when submerged fermentations were performedusing pectin-containing fruit wastes as substrates.None of the strains produced neither lyase nor rhamnogalacturonanhydrolase activities. Regarding pectin esteraseactivity, it was only produced in lower amounts by G. pullulans8E (0.022 U ml−1). A TLC analysis of the substratecleavage pattern of the pectinolytic systems was consistentwith an endo-type activity. The clarification of apple juicewas only accomplished by G. pullulans pectinolytic system,with a clarification of 80% (%T650) using 4 U/ml ofenzyme at 20 °C. As far as we concern this work describes for the first time the production of pectinases by the coldadaptedyeasts species Cystofilobasidium infirmominiatum,Cryptococcus adeliensis and G. pullulans.