CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
artículos
Título:
Effects of the inoculant strain Sphingomonas paucimobilis 20006FA on soil bacterial community and biodegradation in phenanthrene contaminated soil.
Autor/es:
COPPOTELLI B; IBARROLAZA A.; DEL PANNO M; MORELLI I
Revista:
MICROBIAL ECOLOGY
Referencias:
Año: 2008 vol. 55 p. 173 - 183
ISSN:
0095-3628
Resumen:
The effects of the inoculant strain Sphingomonas
paucimobilis 20006FA (isolated from a phenanthrenecontaminated
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
paucimobilis 20006FA (isolated from a phenanthrenecontaminated
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Sphingomonas
paucimobilis 20006FA (isolated from a phenanthrenecontaminated
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
20006FA (isolated from a phenanthrenecontaminated
soil) on the dynamics and structure of
microbial communities and phenanthrene elimination rate
were studied in soil microcosms artificially contaminated
with phenanthrene. The inoculant managed to be established
from the first inoculation as it was evidenced by
denaturing gradient gel electrophoresis analysis, increasing
the number of cultivable heterotrophic and PAH-degrading
cells and enhancing phenanthrene degradation. These
effects were observed only during the inoculation period.
Nevertheless, the soil biological activity (dehydrogenase
activity and CO2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.
2 production) showed a late increase.
Whereas gradual and successive changes in bacterial
community structures were caused by phenanthrene contamination,
the inoculation provoked immediate, significant,
and stable changes on soil bacterial community. In
spite of the long-term establishment of the inoculated strain,
at the end of the experiment, the bioaugmentation did not
produce significant changes in the residual soil phenanthrene
concentration and did not improve the residual
effects on the microbial soil community.