Extracellular enzymes of Fusarium graminearum isolates

KIKOT, GISELE ELEONORA; HOURS, ROQUE; ALCONADA, TERESA

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY

TECPAR cnpq

Lugar: Curitiba; Año: 2008

1516-8913

Fusarium graminearum isolates from three different agroecological regions in Argentina were examined according to the production of different extracellular enzyme activities of potential biotechnological interest: pectinases (PGase: polygalacturonase and PMGase: polymethylgalacturonase), cellulase (CMCase: carboxymethylcellulase) and hemicellulase (xylanase). The isolates were grown in minimum salt medium supplemented with 0.25% glucose, 0.125% citric pectin and 0.125% oat bran as carbon sources and/or enzyme inducers. PGase activity was detected early (after 2 days of incubation) in all the cultures; it was found to be the highest for all the isolates. PMGase was high only for those isolates of the II region. CMCase and endoxylanase activities were particularly found at late stages (after 4 and 7 days of incubation, respectively) and the maximum values were lower than pectinase activities.