CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
artículos
Título:
Iron speciation in dissimilatory Fe(III)-reducing cultures
Autor/es:
C. GARCÍA-BALBOA; M. S. VICENTE; M. L. BLÁZQUEZ; F. GONZÁLEZ; J. A. MUÑOZ; A. BALLESTER
Revista:
GEOMICROBIOLOGY JOURNAL
Editorial:
Taylor and Francis Group
Referencias:
Año: 2009
ISSN:
0149-0451
Resumen:
A protocol has been developed for determination of iron distribution and
speciation in the solid and liquid phases of dissimilatory Fe(III)-reducing bacteria
cultures. Sampling methods and sample processing have been improved. Three different
extraction conditions have been tested for iron measurement: hydrochloric acid,
ammonium oxalate and hydroxylamine hydrochloride. Separate collection of samples
for liquid and solids analysis was tested, providing the best insight into Fe distribution.
One sample was taken from the aqueous phase after solid settlement in vials to
determine the concentration of dissolved iron species; the other was taken from agitated
flasks, in which iron was extracted from solids prior to the analysis. Extraction with
HCl 1M for 24 hours proved the least labor-intensive procedure, with extraction
efficiency adequate for our purposes. The extractant properties of HCl, less aggressive
than the others, dissolved only the bio-available fraction of Fe(III)