Shotgun proteome analysis of Bordetella pertussis reveals a distinct 1 influence of iron 2 availability on the bacterial metabolism, virulence, and defense response

ALVAREZ HAYES, JIMENA; LAMBERTI, YANINA; SURMANN, KRISTIN ; SCHMIDT,FRANK; VÖLKER, UWE; RODRIGUEZ, MARIA EUGENIA

PROTEOMICS (WEINHEIM. PRINT)

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2015 vol. 15 p. 2258 - 2266

1615-9853

One of the mechanisms involved in host immunity is the limitation of iron accessibility to pathogens, which in turn provokes the corresponding physiological adaptation of pathogens. This study reports a gel-free nanoLC-MS/MS-based comparative proteome analysis of Bordetella pertussis grown under iron-excess and iron-depleted conditions. Of the 926 proteins covered 98 displayed a shift in their abundance in response 7 to low iron availability. Forty-seven of them were found to be increased in level while 58 were found with decreased protein levels under iron starvation. In addition to proteins previously reported to be influenced by iron in B. pertussis, we observed changes in metabolic proteins involved in fatty acid utilization and poly-hydroxybutyrate production. Additionally, many bacterial virulence factors regulated by the BvgAS two-component system were found at decreased levels in response to iron limitation. These results, together with the increased production of proteins potentially involved in oxidative stress resistance, seem to indicate that iron starvation provokes changes in B. pertussis phenotype that might shape host-pathogen interaction.Para acceder al Supplemetal materia ir a:http://onlinelibrary.wiley.com/wol1/doi/10.1002/pmic.201400512/full